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Chapitres
- 00:05Titre
- 02:02Procedural Flow Chart
- 03:19cDNA Synthesis
- 06:31End Repair, Paired End Library
- 07:37Adding ‘A’ Bases to the 3′ End of the DNA Fragments
- 08:31Adapter Ligation
- 10:32Size Selection/Gel Purification
- 11:20Evaluation of Single-Read and Paired-End Sequencing Libraries
- 14:18Conclusion
Qui si descrive un metodo per la preparazione di entrambi i singoli letti e associato fine Illumina mRNA-Seq librerie sequenziamento per l'analisi di espressione genica basata su amplificazione lineare dell'RNA T7. Questo protocollo richiede solo 10 nanogrammi di RNA totale di partenza e genera librerie altamente coerente rappresentanza trascrizioni intero.
Tags
MRNA-SeqIllumina LibrariesGlobal Gene ExpressionMutation AnalysisAllele-specific ExpressionGenome-wide AnalysesNon-sequenced GenomesTranscript Copy NumbersIllumina’s Genome AnalyzerPaired End SequencingAlternate Splice JunctionsInsertions And DeletionsDe Novo Transcriptome AssemblyLimited Starting MaterialNanograms Of Total RNAPCR AmplificationMinimal PCR Amplification
