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Chapitres
- 00:05Titre
- 01:36Staining Nematodes with DiI
- 04:24Mounting DiI-stained Nematodes
- 05:40DiI Staining of C. elegans Cuticle
- 07:52Conclusion
Vi presenterer en metode for å visualisere cuticle i live<em> C. elegans</em> Med røde fluorescerende lipofile fargestoff DII (1,1 '-dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine perklorat), som ofte brukes i<em> C. elegans</em> For å visualisere miljømessig utsatt nerveceller. Med denne optimalisert protokollen, alae og ringformet cuticular strukturer farget av DII og observert bruk sammensatte mikroskopi.
Tags
VisualizationCaenorhabditis ElegansCuticular StructuresLipophilic Vital Dye DiICuticleResistant StructureEnvironment ProtectionBody ShapeMotilityEpidermal CellsLipid LayerCircumferential RidgesAnnuliAlaeDevelopment StagesMutationsCollagen OrganizationCompound MicroscopyDIC OpticsFluorescent Transgene ExpressionAntibody StainingElectron MicroscopyWheat Germ Agglutinin (WGA)Cuticular GlycoproteinsFluorescent DyeLive Imaging
