Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Chemical and Biological Engineering,Illinois Institute of Technology, 2Department of Biomedical Engineering,Illinois Institute of Technology, 3Department of Biomedical Engineering,University of California at Irvine, 4Wake Forest Institute for Regenerative Medicine and Department of Biomedical Engineering,Wake Forest University Health Sciences, 5Research Service,Hines Veterans Administration Hospital
Chapitres
- 00:05Titre
- 01:37Alginate Microbead Preparation
- 04:46Coating the Microbeads with Poly-L-ornithine
- 07:59Creating the Outer Alginate Layer
- 09:22Representative Results
Nelle sezioni seguenti, si delineano le procedure per la preparazione di microsfere di alginato per l'uso in applicazioni biomediche. Specificatamente illustrano una tecnica per creare multistrato alginato microsfere per il duplice scopo di incapsulamento delle cellule e proteine come potenziale trattamento di diabete di tipo 1.
Tags
Alginate MicrospheresBiomedical ApplicationsHydrophilic NatureBiocompatibilityPhysical ArchitectureCell EncapsulationDrug DeliveryStem Cell CultureIslets EncapsulationType I Diabetes TreatmentMicrovascular Network FormationOxygen TransportGlucose TransportVital Nutrients TransportFibroblast Growth Factor-1 (FGF-1)Sustained ReleaseGrowth Factor DeliveryBlood Vessel FormationFunctional Graft Outcomes
