Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:34Obtaining and Staging Metamorphic Flies
- 02:49Steps to Remove Specimen from Puparium
- 03:33Confocal Microscopic Evaluation of Reporter Gene Expression in a Whole Mount Sample
- 04:49Quantifying cis-Regulatory Element Activity with ImageJ
- 05:34Utility of Quantitative CRE Activity
- 07:34Conclusion
Fænotypiske variation for egenskaber kan skyldes mutationer i cis-regulerende element (CRE) sekvenser, der kontrollerer genekspression mønstre. Metoder afledt til brug i Drosophila melanogaster kan kvantitativt sammenligne niveauet af rumlige og tidsmæssige mønstre af genekspression medieret ved ændret eller naturligt forekommende CRE varianter.
Tags
Cis-regulatory ElementCRE ActivitiesGene Expression PatternsEnhancersCis-regulatory ModulesTranscription Factor ProteinsRegulatory LogicDevelopment ProgramMorphological EvolutionGenetic VariationPhenotypic VariationReporter TransgenesIn Vivo FunctionHeterologous PromoterCoding SequencesReporter GeneProtein ProductP-element Mediated TransgenesisDrosophila Melanogaster
