Journal
/
Biologie
/
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

DOI:
10.3791/3410-v

•

13:38 min

•

January 27, 2012

•

, , ,
1Department of Cell and Molecular Biology,Karolinska Institutet

Chapitres

  • 00:05Titre
  • 02:19Introducing the Probe to Cells
  • 03:27Monitoring Ratiometric FRET
  • 06:12Verifying and Optimizing Conditions
  • 06:43Analyzing FRET
  • 11:24Representative Imaging Results
  • 12:52Conclusion

Summary

Traduction automatique

Traduction automatique

FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.

Tags

KinasePhosphataseCell CycleRatiometric FRETFörster Resonance Energy TransferFRET-based ReportersCFPYFPPhosphorylationConformation ChangeDistanceOrientationFRET EfficiencyProbesPKAPKBPKCPKDERKJNKCdk1Aurora BPlk1Stress Signaling PathwaysProgression Through The Cell CycleUnperturbed GrowthRecovering From StressTime-lapse ImagingSignal To Noise Ratio

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/3410?article_type=v

Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
check_url/fr/3410?article_type=v

Analysis of Cell Cycle Position in Mammalian Cells
check_url/fr/3410?article_type=v

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
check_url/fr/3410?article_type=v

Studying DNA Looping by Single-Molecule FRET
check_url/fr/3410?article_type=v

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
check_url/fr/3410?article_type=v

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
check_url/fr/3410?article_type=v

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
check_url/fr/3410?article_type=v

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
check_url/fr/3410?article_type=v

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
check_url/fr/3410?article_type=v

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Read Article