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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
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Journal JoVE Biologie
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

DOI:
10.3791/3442-v

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09:40 min

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February 13, 2012

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1Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center

Chapitres

  • 00:05Titre
  • 01:24Preparation of Bacteria with Library Clones
  • 03:14Preparation of Nematodes
  • 06:51Observation of Nematodes
  • 08:55Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.

Tags

RNAi ScreeningC. ElegansPostembryonic PhenotypesModel SystemGenesGene PathwaysFunctional CharacterizationProtocolRNAi ScreenPhenotype AssayFluorescent ProductTransgeneGenomic RNAi LibraryCDNA InsertsRNAi-sensitive BackgroundHatched Animals

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