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Chapitres
- 00:05Titre
- 01:01Fission Yeast Culture and Sample Preparation
- 03:28Microscopy and Quantitative Measurements
- 05:03Representative Measurements in Yeast
- 06:27Conclusion
Den fission gær,<em> Schizosaccharomyces pombe</em>, Er en god model system for at studere de grundlæggende cellulære processer. Her beskriver vi en metode til at udføre kvantitativ levende celler analyse af fission gær. I dette særlige eksperiment har vi fokus på organisering af genomet i cellekernen, men metoden kan også bruges til at studere cytosolisk faktorer.
Tags
Quantitative Live Cell Fluorescence-microscopy AnalysisFission YeastMicroscopy TechniquesSpecific Protein DetectionLive Cell ImagingFluorochromesGreen Fluorescent Protein (GFP)Subcellular LocalizationsProtein MovementsFluorescent MicroscopeSubnuclear LocalizationChromosome StudyLac Repressor Protein (LacR)LacO SequenceGenome VisualizationHomologous RecombinationTargeted IntegrationEngineered Fusion Proteins
