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Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

DOI:
10.3791/3466-v

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12:03 min

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February 21, 2012

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, , , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Center for Neural Development and Disease,University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease,University of Rochester Medical Center

Chapitres

  • 00:05Titre
  • 01:42Preparing the Animal for IMaging
  • 02:56Preparing the Open Skull Cranial Window
  • 06:40Intravenous Injection and Blood Oxygenation Monitoring
  • 07:58Two-photon Imaging
  • 09:30Representative Hypoxia Measurement Results
  • 11:14Conclusion

Summary

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Traduction automatique

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

Tags

DetectionMicroregional HypoxiaMouse Cerebral CortexTwo-photon ImagingEndogenous NADH FluorescenceOxygen SupplyBlood FlowVisualized Experimental ProtocolMethodological ProtocolPerivascular Oxygen GradientsNon-linear RelationshipNicotinamide Adenine Dinucleotide (NADH)Fluorescence IntensityOxygen Partial PressureTwo-photon ExcitationTexas-Red DextranAdvantagesStandard Two-photon In Vivo Imaging EquipmentContinuous MonitoringDepth ResolutionVulnerable Watershed AreasDecline In Vascular Oxygen SupplyMicroregional Cortical Oxygenation

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