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Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
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Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

DOI:
10.3791/3482-v

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05:05 min

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November 04, 2011

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, , ,
1Department of Radiology,Molecular Imaging Program at Stanford (MIPS) , 2Stanford School of Medicine,Stanford University

Chapitres

  • 00:05Titre
  • 01:25Superparamagnetic Iron Oxide
  • 01:59Labeling Human Mesenchymal Stem Cells
  • 02:39Prepare Labeled Cells
  • 03:31Ferumoxytol: Iron Oxide Nanoparticle
  • 04:30Conclusion

Summary

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We describe a technique for labeling and tracking stem cells with FDA-approved, superparamagnetic iron oxide (SPIO), ferumoxytol (Feraheme). This cellular imaging technique that utilizes magnetic resonance (MR) imaging for visualization, is readily accessible for long-term monitoring and diagnosis of successful or unsuccessful stem cell engraftments in patients.

Tags

Stem CellsRegenerative MedicineIn Vivo KineticsTransplanted CellsNon-invasive MethodMonitor Stem Cell TransplantsEngraftment OutcomesHEK293 CellsHMSCsIPS CellsMultipotencyDifferentiationOsteoblastsAdipocytesChondrocytesPluripotent

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