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Assaying the Kinase Activity of LRRK2 in vitro
Journal JoVE
Biologie
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Journal JoVE Biologie
Assaying the Kinase Activity of LRRK2 in vitro

Assaying the Kinase Activity of LRRK2 in vitro

DOI:
10.3791/3495-v

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06:09 min

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January 18, 2012

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1Department of Molecular Neuroscience,UCL Institute of Neurology

Chapitres

  • 00:05Titre
  • 01:18Preparing the Kinase Reactions and Running the Assay
  • 03:30Transferring the Samples and Analyzing Results
  • 04:45Results: Kinase Assay
  • 05:49Conclusion

Summary

Traduction automatique

Traduction automatique

Leucine Rich Repeat Kinase 2 is a large multidomain kinase, mutations in which are the most common genetic cause of Parkinson's disease. Analysis of the kinase activity of this protein has proven to be a crucial tool in understanding the biology and dysfunction of this protein. In this paper, in vitro assaying of the kinase activity of LRRK2 and a selection of its mutants is described, providing an experimental system to examine phosphorylation of putative substrates and potential dysfunction of LRRK2 in disease.

Tags

AssayingKinase ActivityLRRK2In VitroLeucine Rich Repeat Kinase 2ROCO Family Of ProteinsGTPase DomainKinase DomainParkinson’s DiseaseEnzymatic ActivitiesCell DeathMutationsInhibitorsExperimental ToolEnzymatic PropertiesDrug TargetsApproachesEpitope Tagged ProteinMammalian Cell LinesImmunoprecipitated

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