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Intravital Imaging of the Mouse Thymus mittels 2-Photonen-Mikroskopie
Journal JoVE
Immunologie et infection
Author Produced
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Journal JoVE Immunologie et infection
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
DOI:

10:08 min

January 07, 2012

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Chapitres

  • 00:01Titre
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Résultats

Summary

Traduction automatique

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

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