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Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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Journal JoVE Biologie
Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

DOI:
10.3791/3511-v

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07:33 min

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December 21, 2011

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, , ,
1Department of Biochemistry,Temple University , 2Department of Anesthesiology and Pain Medicine,University of Washington

Chapitres

  • 00:05Titre
  • 01:18Oxidation-sensitive Dyes: Visualization of ROS in J774 Cells
  • 02:24Fluorescent Sensor HyPer: Visualization of ROS in Stably Transfected MPMVECs
  • 04:04Co-Culture Model for Paracrine-Derived ROS
  • 05:13ROS Dynamics
  • 06:50Conclusion

Summary

Traduction automatique

Traduction automatique

An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.

Tags

Vascular Ca2+ SignalingParacrineROS (reactive Oxygen Species)Oxidative StressIschemia/reperfusion DamageSepsisSignal TransductionCell ProliferationCell DeathInflammatory CellsCytokinesTumor Necrosis Factor-alpha (TNF-alpha)IL-1Endothelial Cells (EC)Endothelial DysfunctionMacrophagesNADPH Oxidases

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