Journal
/
Neurosciences
/
Whole Animal Perfusion Fixation for Rodents
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Whole Animal Perfusion Fixation for Rodents

Whole Animal Perfusion Fixation for Rodents

DOI:
10.3791/3564-v

•

08:53 min

•

July 30, 2012

•

, ,
1Biomedical Engineering,University of Michigan , 2Department of Neurological Surgery,University of Washington School of Medicine

Chapitres

  • 00:05Titre
  • 01:12Prepare Apparatus
  • 02:58Perfusion Surgery
  • 05:08Perfusion
  • 06:28Brain Dissection and Storage
  • 08:40Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.

Tags

Whole Animal Perfusion FixationRodentsFixationImmersion FixationHypoxiaPerfusing FixativeCirculatory SystemVascular NetworkPhysiological PressuresPerfusion Fixation TechniquesParaformaldehyde PerfusionBrain PreservationImmunohistochemistry

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Automated Interactive Video Playback for Studies of Animal Communication

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

A Low Cost Setup for Behavioral Audiometry in Rodents

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Deep Brain Stimulation with Simultaneous fMRI in Rodents

Acrylic Resin Molding Based Head Fixation Technique in Rodents

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Cannula Implantation into the Cisterna Magna of Rodents

Read Article