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Bedöma Somatisk Hypermutation i Ramos B-celler efter överuttryck eller Knockdown av specifika gener
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Chapitres
- 00:05Titre
- 01:00Transfecting BOSC 23 Cells and Harvesting Viral Particles
- 02:27Infecting B Cells
- 03:34Single Cell Seeding
- 04:42Analysis: IgM Loss (3 Weeks and 5 Weeks)
- 05:57Representative Effects of Overexpressing Activated-induced Cytidine Deaminase (AID) or Knocking Down a Repair Factor in Ramos Cells
- 07:48Conclusion
Tags
Somatic HypermutationRamos B CellsOverexpressionKnockdownSpecific GenesV(D)J RecombinationAffinity AntibodiesImmunoglobulin GeneSomatic Hypermutation (SHM)Class Switch Recombination (CSR)Activation-induced Cytidine Deaminase (AID)Cytosine ResiduesUracilsRepair PathwaysMolecular DetailsMice ModelsGenetic KnockoutsRepair FactorsDouble-strand Break Repair ProteinsB-cell Development
