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Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
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Chapitres
- 00:05Titre
- 01:26Incubate Chick and Quail Eggs to the Desired Stage
- 01:57Prepare Eggs for Windowing and Dissection
- 06:15Grafting the Tissue
- 07:21Immunofluorescent Detection of Quail-specific Nuclear Antigen QCPN in Donor-derived Cells in the Chimeric Embryo
- 08:35Conclusion
An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.
