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Konstruktion af et Collagen hydrogel til levering af stamcelle-loaded chitosanmikrosfærer
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Chapitres
- 00:05Titre
- 01:25Isolating Adipose-derived Stem Cells
- 02:40Preparing Chitosan Microspheres
- 04:00Quantify Free Amino Groups in CSM
- 05:20Loading ASC in CSM
- 06:11Quantify ASC Loading and Cell Viability in CSM
- 07:17Characterizing ASC-CSM-embedded Collagen Gel
- 08:04ASC-CSM-collagen Gel
- 08:55Conclusion
En stor hurdle i de nuværende stamceller er at bestemme den mest effektive metode til at levere disse celler til værtsvævene. Her beskriver vi en chitosan-baserede leveringsmetode som er effektiv og enkel fremgangsmåde, samtidig med at adipøst afledte stamceller for at bevare deres multipotency.
Tags
Collagen HydrogelStem Cell-loaded Chitosan MicrospheresRegenerative MedicineImplantation SiteMechanical PropertiesVascularizationExtracellular MatrixBiocompatible MaterialsChemical CompositionMechanical StrengthPermeabilityOxygen And NutrientsChitosanCationic PolysaccharideChemical ModificationIn Vivo MacromoleculesGlycosaminoglycan PortionSubstrate For Cell AdhesionMigration And ProliferationAdipose-derived Stem Cells (ASC)Three-dimensional Scaffold
