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Chapitres
- 00:05Titre
- 01:20Perfusion of the Liver
- 03:31Preparation of the Biliary Tree and Isolation of the Portal Fibroblast Fraction
- 06:42Results: Representative Primary Portal Fibroblasts Isolated from Adult Rat Liver
- 07:21Conclusion
A technique for isolating portal fibroblasts from rat liver is described. Livers are perfused and digested in situ with collagenase, followed by ex vivo digestion of the liver slurry and size selection of cells. This method provides a pure population of portal fibroblasts without the need for passage in culture.
Tags
IsolationRat Portal FibroblastsIn Situ Liver PerfusionLiver FibrosisExtracellular MatrixActivated MyofibroblastsHepatic Stellate CellsBone Marrow Derived FibroblastsBiliary FibrosisBiliary Epithelial InjuryScarringIn Vitro StudyCell PopulationIsolation TechniqueOutgrowth TechniqueLiver PerfusionEnzymatic DigestionSize SelectionPrimary Cells
