This content is Free Access.
Chapitres
- 00:05Titre
- 01:22Drosophila Ovary Dissection
- 02:30Isolating Ovaries
- 04:32Isolating Individual Late-stage Egg Chambers
- 05:38Injection Preparation
- 09:28Images of Dissected and Injected Oocytes
- 10:10Conclusion
The Drosophila egg chamber is an excellent model for studying the mechanisms of mRNA localization. In order to capture the dynamic events that underpin the processes of localization, rapid high resolution imaging of live tissue is required. Here, we present a protocol for dissection and imaging of live samples with minimal disruption.
Tags
DrosophilaLive ImagingPreparationEgg ChambersPhysiological RelevanceImaging ConditionsDehydrationHypoxiaOverheatingMedium DeteriorationBody PatterningMRNA LocalizationCytoskeletal OrganizationHalocarbon OilFluorescence ProteinsTransgenesLabeled RNAProtein AntibodiesMS2 ConstructsGenomeOocyte Extraction
