Journal
/
Immunologie et infection
/
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

DOI:
10.3791/3683-v

•

11:31 min

•

August 08, 2012

•

, ,
1Department of Immunology,College of Medicine, Mayo Clinic

Chapitres

  • 00:05Titre
  • 01:16Generation of Short-term Human Antigen-specific CD8 T Cell Lines
  • 02:55Preparation of Breast Cancer Cells
  • 04:19Co-culture of Tumor with CD8 T Cells
  • 05:45Chromium Release Assay (CRA)
  • 07:21HER-2/neu p369-specific T Cell / SKBR3 Breast Cancer Cells Co-cultures
  • 11:09Conclusion

Summary

Traduction automatique

Traduction automatique

The chromium release assay, a common assay for detecting cytotoxic T cell activity, has several limitations. Using antigen-specific CD8 T cells and the human breast cancer tumor line, SKBR3, in the present article, an impedance-based approach was examined for the capability of detecting cell killing.

Tags

Cytotoxic ActivityCD8 T CellsCr51 Release AssayXCELLigence SystemT Cell Receptor (TCR)Antigen-specific CD8 T CellsELIspotFlow CytometryCytotoxicity AssayChromium Release Assay (CRA)Gamma RadiationReproducibilityCaspase ReleaseBLT Esterase ActivityCD107 ExpressionImpedance-based AssayRoche XCelligence System

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/3683?article_type=v

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
check_url/fr/3683?article_type=v

Determining the Phagocytic Activity of Clinical Antibody Samples
check_url/fr/3683?article_type=v

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
check_url/fr/3683?article_type=v

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
check_url/fr/3683?article_type=v

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
check_url/fr/3683?article_type=v

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
check_url/fr/3683?article_type=v

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
check_url/fr/3683?article_type=v

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
check_url/fr/3683?article_type=v

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
check_url/fr/3683?article_type=v

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Read Article