/
/
קביעת פעילות ציטוטוקסית אופטימלית של CD8 תאי T ספציפי אדם Her2neu על ידי השוואת Assay שחרור Cr51 למערכת xCELLigence
This content is Free Access.
Chapitres
- 00:05Titre
- 01:16Generation of Short-term Human Antigen-specific CD8 T Cell Lines
- 02:55Preparation of Breast Cancer Cells
- 04:19Co-culture of Tumor with CD8 T Cells
- 05:45Chromium Release Assay (CRA)
- 07:21HER-2/neu p369-specific T Cell / SKBR3 Breast Cancer Cells Co-cultures
- 11:09Conclusion
Tags
Cytotoxic ActivityCD8 T CellsCr51 Release AssayXCELLigence SystemT Cell Receptor (TCR)Antigen-specific CD8 T CellsELIspotFlow CytometryCytotoxicity AssayChromium Release Assay (CRA)Gamma RadiationReproducibilityCaspase ReleaseBLT Esterase ActivityCD107 ExpressionImpedance-based AssayRoche XCelligence System
