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Chapitres
- 00:05Titre
- 02:19Printing the Antibody Microarray
- 03:58Chemically Blocking the Antibody Microarray
- 06:06Blocking Non-specific Binding with BSA
- 06:47Wax Imprinting
- 07:11Applying Serum Samples
- 08:19Applying Biotinylated Glycan-binding Protein
- 09:00Applying Dye-labeled NeutrAvidin and Fluorescence Detection
- 10:03Data Extraction and Analysis
- 12:08Conclusion
I denne undersøgelse beskriver vi en forbedret protokol til et multiplekset high-throughput-antistof mikroarray med lectin påvisningsmetode, der kan anvendes i glycosylering profilering af specifikke proteiner. Denne protokol indeholder nye pålidelige reagenser og reducerer den tid, omkostninger og lab krav til udstyr i forhold til den tidligere procedure.
Tags
Chemically-blocked Antibody MicroarrayMultiplexed High-throughput ProfilingSpecific Protein GlycosylationComplex SamplesGlycan Binding Protein DetectionPost-translational ModificationPhysical Properties Of ProteinsChemical Properties Of ProteinsBiological Properties Of ProteinsDisease ProgressionMalignant TransformationEarly Detection BiomarkersCancer BiomarkersPractical MethodLaboratory SettingsClinical SettingsChemically Blocked Antibody MicroarrayGlycan-binding Protein DetectionTime ReductionCost ReductionLab Equipment Requirements
