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Immunology and Infection

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JoVE Journal Immunology and Infection
En primær Neuron Kultur System for studier av herpes simplex virus ventetid og reaktivering
 
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En primær Neuron Kultur System for studier av herpes simplex virus ventetid og reaktivering

Article DOI: 10.3791/3823-v • 12:22 min • April 2nd, 2012
April 2nd, 2012 •
1, 1, 2, 3, 2,4,5,6,7, 1, 1
1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

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Protokollen beskriver en effektiv og reproduserbar modellsystem for å studere herpes simplex virus type 1 (HSV-1) ventetid og reaktivering. Analysen benytter homogene sympatiske nevroner kulturer og åpner for molekylær disseksjon av virus-Nevron interaksjoner med en rekke verktøy, inkludert RNA interferens og uttrykk av rekombinante proteiner.

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Immunologi nevron cellekultur herpes simplex virus (HSV) molekylær biologi virologi
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