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Dyrking av Human Neural stamceller i en 3-dimensjonal Self-montering Peptid Hydrogel
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Chapitres
- 00:05Titre
- 01:09Culture of hNPCs in a PuraMatrix
- 03:41Immunocytochemical Staining of Entire Matrices
- 05:44Release of hNPCs from the Scaffolds for Flow-cytometry Analysis
- 07:04Quantification of βIII-tubulin Positive Cells by Flow Cytometry
- 08:09Observation and Enumeration of PuraMatrix Encapsulated hNPCs
- 10:37Conclusion
Her beskriver vi bruk av en selv-montering 3-dimensjonale stillas til kultur menneskelige nevrale stamceller. Vi presenterer en protokoll for å frigjøre cellene fra stillasene for å bli analysert senere for eksempel ved flowcytometri. Denne protokollen kan tilpasses andre celletyper å utføre detaljerte mechanistically studier.
Tags
CultivationHuman Neural Progenitor Cells3-dimensionalSelf-assembling Peptide HydrogelGrowthProliferationNeuronal DifferentiationNeurological DisordersNeurodegenerative DiseasesBiomaterial ScaffoldsRegenerative MedicineStem CellsProgenitor CellsCulture Systems3D EnvironmentSurvivalFatePuraMatrixPeptide Hydrogel Scaffold3D-culture SystemNano-fibersXeno-freePM-concentration
