Journal
/
Biologie
/
Measurement of Cellular Chemotaxis with ECIS/Taxis
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Measurement of Cellular Chemotaxis with ECIS/Taxis

Measurement of Cellular Chemotaxis with ECIS/Taxis

DOI:
10.3791/3840-v

•

11:37 min

•

April 01, 2012

•

, , ,
1Molecular and Cell Biology,University of Connecticut, 2University of Connecticut

Chapitres

  • 00:05Titre
  • 01:28ECIS/Taxis Electrode Preparation
  • 03:13Preparing Agarose Chambers and the Cannula Well-cutting Tool
  • 04:21Cutting Wells into Agarose
  • 05:51Loading the Wells
  • 07:04Data Collection
  • 08:55Data Management
  • 10:13Results: Chemotaxis of Human Jurkat T cells in Response to SDF1alpha
  • 11:14Conclusion

Summary

Traduction automatique

Traduction automatique

The ECIS/Taxis system is an automated, real-time assay that measures cellular chemotaxis. In this assay, cells move beneath a layer of agarose to arrive at a target electrode. Cellular movement is measured by the onset of resistance to AC current 0.

Tags

Cellular ChemotaxisECISTaxisCellularBoyden Chamber AssayUnder-agarose Chemotaxis AssayChemoattractant GradientSolid SubstrateAqueous FilmAgarose LayerAutomated ECIS/Taxis SystemElectric Cell-substrate Impedance SensingTarget ElectrodesCounter-electrode

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/3840?article_type=v

Studies of Bacterial Chemotaxis Using Microfluidics – Interview
check_url/fr/3840?article_type=v

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
check_url/fr/3840?article_type=v

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
check_url/fr/3840?article_type=v

Identification of protein complexes with quantitative proteomics in S. cerevisiae
check_url/fr/3840?article_type=v

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
check_url/fr/3840?article_type=v

Measurement Of Neuromagnetic Brain Function In Pre-school Children With Custom Sized MEG
check_url/fr/3840?article_type=v

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
check_url/fr/3840?article_type=v

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
check_url/fr/3840?article_type=v

Measurement of Lifespan in Drosophila melanogaster
check_url/fr/3840?article_type=v

C. elegans Chemotaxis Assay

Read Article