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Genotyping of Plant and Animal Samples without Prior DNA Purification
Journal JoVE
Biologie
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Journal JoVE Biologie
Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

DOI:
10.3791/3844-v

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11:00 min

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September 24, 2012

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, , , ,
1Thermo Scientific Molecular Biology Products,Thermo Fisher Scientific

Chapitres

  • 00:05Titre
  • 01:48Genotyping of Arabidopsis Plant Individuals with Direct Protocol
  • 04:23Amplifying Specific DNA Fragments from 15-year-old Oak Leaves using the Dilution Protocol
  • 05:50Genotyping of Transgenic Mice using the Dilution Protocol
  • 07:05Results: Direct PCR Analysis
  • 10:30Conclusion

Summary

Traduction automatique

Traduction automatique

The Direct PCR approach presented here facilitates PCR amplification directly from small amounts of unpurified plant and animal tissue.

Tags

GenotypingPlant SamplesAnimal SamplesDNA PurificationDirect PCR ApproachThermo Scientific PhusionThermo Scientific Phire DNA PolymerasesPCR-based Target DNA DetectionPlant ResearchGenotype AnalysisTransgenesDNA IsolationThermo Scientific Phire Plant Direct PCR KitArabidopsis Plant LeavesCleaved Amplified Polymorphic Sequence Analysis (dCAPS)Single Nucleotide Polymorphisms (SNPs)Restriction Endonuclease DigestionPhenolic Compounds

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