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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
Journal JoVE
Biologie
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Journal JoVE Biologie
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

DOI:
10.3791/3851-v

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13:34 min

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September 29, 2012

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, , , , ,
1Molecular Microbiology and Immunology,University of Missouri, 2Department of Surgery,University of Missouri, 3Child Health,University of Missouri

Chapitres

  • 00:00Titre
  • 01:43Harvesting RNP Complex
  • 03:59Preparation of Isolation Materials
  • 06:40Immunoprecipitation
  • 08:42DNase and Proteinase K Treatment and RNA Precipitation
  • 12:11Representative Results
  • 12:49Conclusions

Summary

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A step by step protocol to isolating and identifying RNA associated complexes through RIP-Chip.

Tags

IsolationIdentificationMRNAsMicroRNAsProtein ComponentsRibonucleoprotein ComplexesRIP-ChipHigh-throughput SequencingMicroarray AnalysisGlobal Gene Expression AnalysisPost-transcriptional Gene RegulationRNA Binding Proteins (RBP)MRNA LocalizationStabilityTranslationRibonucleoprotein (RNP) ComplexDe Novo MRNA TargetsCellular ExtractsProtein OutputRNP Immunoprecipitation-microarray (RIP-Chip)Experimental ConditionsPost-transcriptional Gene Regulation MechanismsRibonucleoprotein Interactions

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