Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:55Preparing the STLV Bioreactor
- 04:01Preparing Microcarrier Beads
- 04:35Seeding Epithelial Cells and Beads into the STLV Bioreactor
- 06:45Culturing, Sampling, and Photo Documentation of Cultures
- 08:19Transferring and Harvesting Aggregates
- 09:273D-aggregates and Biology of Human Vaginal Epithelial Cells
- 11:59Conclusion
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
Tags
Rotating Wall Vessel Bioreactor3D Epithelial Cell ModelsIn Vitro Cell CultureGrowth EnvironmentConventional Cell Culture SystemsPhysiologically Relevant MicroenvironmentRotating Wall Vessel (RWV) Bioreactor TechnologyOrganotypic ModelsExternal StimuliSuspension Culture SystemLow Physiological Fluid Shear ConditionsHigh-aspect Rotating Vessel (HARV)Slow-turning Lateral Vessel (STLV)Aeration Source
