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Chapitres
- 00:05Titre
- 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
- 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
- 09:01Magnetic Enrichment of Gr-1+ Leukocytes
- 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
- 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
- 13:40Conclusion
Dette er en hurtig og omfattende fremgangsmåden immunfænotypebestemmelse Myeloide afledte suppressorceller (MDSC) og berige Gr-1<sup> +</sup> Leukocytter fra mus milte. Denne metode anvender flowcytometri og AutoMACS Cell sortering for at berige for levedygtige Gr-1<sup> +</sup> Leukocytter før FACS-sortering af MDSC til anvendelse<em> In vivo</em> Og<em> In vitro</em> Assays.
Tags
Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes
