/
/
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:45Preparation of Cells
- 02:38Pulling Glass Micropipettes
- 03:53Microaspiration and Image Acquisition
- 07:17Analysis
- 07:46Results: Factors That Control Membrane Deformability
- 10:03Conclusion
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
Tags
Micropipette AspirationSubstrate-attached CellsCell StiffnessBiomechanical PropertiesCellular DeformabilityCellular StiffnessCell ContractionForce GenerationMembrane DeformationNegative PressureGlass MicropipetteMicropipette Aspiration TechniqueMicroaspirationGlass CapillaryPneumatic Pressure TransducerMembrane ProjectionExperimental Approach
