Journal
/
Immunologie et infection
/
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

DOI:
10.3791/3892-v

•

16:10 min

•

March 22, 2012

•

, ,
1Laboratory of Cellular and Molecular Immunology,National Institute of Allergy and Infectious Diseases, National Institutes of Health

Chapitres

  • 00:05Titre
  • 02:59Transfection Using Amaxa Mouse T Cell Nucleofector Kit
  • 06:07TIRF Setup
  • 10:24Imaging
  • 13:19Representative Results
  • 14:54Conclusion

Summary

Traduction automatique

Traduction automatique

The compartmentalization of proteins either within the plasma membrane or into intracellular locations is one regulatory mechanism that can greatly influence signaling outcomes; hence, to understand signaling it is important to study the spatial and temporal behavior of the proteins involved. We describe here a TIRF microscopy based system to study signal transduction in T cells, but is broadly applicable.

Tags

TIRF MicroscopyReal-time ImagingTCRSignaling ProteinsAntigen Presenting CellsPMHC ProteinsTCR ComplexCD3 DimersSrc Family KinaseLckSyk Family KinaseZap70Adapter ProteinsLATSLP76Proximal Signaling ComplexCalcium-dependent Transcription Factor ActivationGene Expression ProgramsT Cell DifferentiationAntigen PotencyCo-stimulatory ReceptorsCo-inhibitory ReceptorsChemokine ReceptorsCytokine Receptors

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/3892?article_type=v

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
check_url/fr/3892?article_type=v

Real-time Live Imaging of T-cell Signaling Complex Formation
check_url/fr/3892?article_type=v

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
check_url/fr/3892?article_type=v

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
check_url/fr/3892?article_type=v

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
check_url/fr/3892?article_type=v

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
check_url/fr/3892?article_type=v

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
check_url/fr/3892?article_type=v

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
check_url/fr/3892?article_type=v

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
check_url/fr/3892?article_type=v

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Read Article