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Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
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1Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention, 2Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention
Chapitres
- 00:05Titre
- 02:02Hemolymph Collection for Slide Preparation
- 03:11Salivary Gland Removal
- 04:38Saliva Collection
- 07:06Representative Results
- 07:52Conclusion
The collection of infected tick hemolymph, salivary glands, and saliva is important to study how tick-borne pathogens cause disease. In this protocol we demonstrate how to collect hemolymph and salivary glands from feeding Ixodes scapularis nymphs. We also demonstrate saliva collection from female I. scapularis adults.
Tags
SalivaSalivary GlandHemolymph CollectionIxodes Scapularis TicksTick-borne IllnessesLyme DiseaseTick-borne Relapsing FeverRocky Mountain Spotted FeverEhrlichiosisAnaplasmosisEncephalitisBabesiosisColorado Tick FeverTularemiaBorrelia Spp.Rickettsia RickettsiiEhrlichia ChaffeensisE. EquiAnaplasma PhagocytophilumTick-borne Encephalitis VirusBabesia Spp.ColtivirusFrancisella TularensisGene Expression RegulationTick Proteins InteractionTick MigrationBorrelia BurgdorferiEnzootic CycleHemostatic ResponseInnate Immune ResponseTick Saliva Proteins
