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Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
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Journal JoVE Biologie
Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks

DOI:
10.3791/3903-v

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05:47 min

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May 11, 2012

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, ,
1Molecular and Microbiology Department and Center for the Study of Genomics in Liver Diseases,George Mason University, 2Translational Research Institute,Inova Health System, 3Research Center for Medical Genetics RAMS

Chapitres

  • 00:05Titre
  • 01:02Assembly of Cryotolerant Device for Retrieval of Cryoboxes
  • 02:15Assembly of Cryotolerant Device for Retrieval of Cryovials
  • 03:12Retrieving Cryoboxes and Cryovials from Dewar
  • 04:12Results: RNA Sample Quality from Retrieved Samples
  • 05:27Conclusion

Summary

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Here we present a low cost, durable cryotolerant device for sample retrieval from Dewar tanks filled with liquid nitrogen. The ease of construction and modular design of the device makes the process of sample retrieval from cryogenic tanks safe and easy.

Tags

Do-It-Yourself DeviceRecoveryCryopreserved SamplesCryogenic Storage TanksLiquid NitrogenSample PreservationCryogenic BurnFrostbiteDewarsOxygen ConcentrationAsphyxiaBiological SamplesCryovialsCryoboxesStainless Steel RacksRetrievalLiquid Nitrogen Filled TankSunken Sample Retrieval

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