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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
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Biologie
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Journal JoVE Biologie
Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

DOI:
10.3791/3928-v

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11:39 min

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September 21, 2012

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, , ,
1Department of Cellular and Molecular Medicine,University of California, San Diego , 2Biosecurity and Public Health,Los Alamos National Laboratory

Chapitres

  • 00:05Titre
  • 01:21Tissue Embedding in OCT
  • 02:13Tissue Sectioning
  • 02:54Tissue Staining with Alcian Blue
  • 03:43Tissue Staining with Periodic Acid Schiff
  • 04:36Tissue Staining with Lectins
  • 05:44Controlling for Lectin Staining Specificity
  • 06:52Results: Mucin Staining in Human and Animal Tissue Sections
  • 11:15Conclusion

Summary

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Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

Tags

Natural Mucin DistributionMucinsGlycoproteinsMucus LayerHost Innate ImmunityAntimicrobial PeptidesCytokinesImmunoglobulinsHistological MethodsStainingFormalin FixationParaffin EmbeddingCryo-protectant Media

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