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Isolation of Immune Cells from Primary Tumors
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Biologie
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Journal JoVE Biologie
Isolation of Immune Cells from Primary Tumors

Isolation of Immune Cells from Primary Tumors

DOI:
10.3791/3952-v

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00:00 min

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June 16, 2012

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, , ,
1Tumor Immunity and Tolerance Section, Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program,National Cancer Institute – Frederick

Summary

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In this report, we describe a protocol for isolating highly purified populations of leukocytes that infiltrate tumors. This protocol is adapted from the Miltenyi Biotech protocol to enhance yield and purity for isolating cells from complex tumor tissue.

Tags

IsolationImmune CellsPrimary TumorsImmunosuppressive MicroenvironmentLeukocytesChemokinesGrowth FactorsTumor-associated LeukocytesTumor-draining Lymph NodesSpleenCell ActivationTraffickingDendritic CellsPhenotypeAdoptively-transferred T CellsTolerizedProliferationCytokine ProductionTumor MicroenvironmentCD4+ T Helper CellsCD8+ T Cells

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