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Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
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Médecine
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Journal JoVE Médecine
Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model

DOI:
10.3791/3996-v

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10:40 min

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August 04, 2012

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1Department of Applied Physics and Physico-Informatics,Keio University, 2Department of Biochemistry, School of Medicine,Keio University, 3Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project,Japan Science and Technology Agency (JST)

Chapitres

  • 00:05Titre
  • 02:44Labeling Erythrocytes with Fluorescein Isothiocyanate Isomer (FITC)
  • 04:11Preparation of Oxygen-sensitive Dye
  • 04:54Animal Preparation
  • 05:58Blood Flow Visualization
  • 06:15pO2 Measurement
  • 08:04Hepatitis Model
  • 08:31Representative Results
  • 10:12Conclusion

Summary

Traduction automatique

Traduction automatique

An optical system was developed to visualize hepatic microcirculation with FITC-labeled erythrocytes and to measure the partial pressure of oxygen in the microvessels with laser-assisted phosphorimetry. This method can be used to investigate physiological and pathological mechanisms by analyzing microvascular structure, diameter, blood flow velocity, and oxygen tension.

Tags

Blood FlowOxygen ConsumptionHepatic MicrocirculationAcute HepatitisOxygen SupplyOxygen DemandPortal Vein BloodGastrointestinal TractSpleenHepatocytesSinusoidsOxygen GradientEnzymesOxygen MetabolismFITC-labeled ErythrocytesLaser-assisted PhosphorimetryPartial Pressure Of OxygenBlood Flow VelocitiesVessel DiametersHepatocyte Necrosis

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