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Generation of Mice Derived from Induced Pluripotent Stem Cells
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Biologie
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Journal JoVE Biologie
Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

DOI:
10.3791/4003-v

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11:56 min

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November 29, 2012

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, , , , , ,
1Dorris Neuroscience Center & Department of Cell Biology,The Scripps Research Institute , 2Mouse Genetics Core Facility,The Scripps Research Institute

Chapitres

  • 00:05Titre
  • 02:12Preparation of Lentivirus
  • 03:24Derivation of iPSC Lines
  • 04:37Generation of Tetraploid Blastocysts
  • 07:20Preparation of iPSCs for Blastocyst Injection
  • 09:20Results: Reprogramming MEFs to iPSCs
  • 10:55Conclusion

Summary

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Generating induced pluripotent stem cell (iPSC) lines produces lines of differing developmental potential even when they pass standard tests for pluripotency. Here we describe a protocol to produce mice derived entirely from iPSCs, which defines the iPSC lines as possessing full pluripotency1.

Tags

Induced Pluripotent Stem CellsIPSCsBasic ResearchRegenerative TherapiesSomatic CellsGerm LayersEmbryoid BodiesTeratomasGene ExpressionChimeric MicePluripotency MarkersDonor Cell SourcesSelection MethodsTetraploid Embryo ComplementationFull PluripotencyElectrofusionBlastocyst Stage

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