Journal
/
Médecine
/
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

DOI:
10.3791/4016-v

•

13:56 min

•

November 28, 2012

•

, ,
1St. Erik’s Eye Hospital,Karolinska Institutet, 2Gullstrand lab, Section for Ophthalmology, Department of Neuroscience,Uppsala University

Chapitres

  • 00:05Titre
  • 02:00Exposure to Ultraviolet Radiation
  • 03:26Dissection
  • 04:31Quantitative RT-PCR
  • 08:56Immunohistochemical Staining
  • 10:06Sectioning and Fluorescence Immunohistochemistry
  • 11:41Representative qPCR and Imaging of UVR Exposed vs Non-exposed Lens
  • 13:30Conclusion

Summary

Traduction automatique

Traduction automatique

Cataract is the leading cause of blindness in the world. Solar ultraviolet radiation (UVR) is the main risk factor for cataract development. An animal model of far UVR-B induced cataract was developed. In this article we describe methods for investigation of cataract formation: exposure to UVR, quantitative RT-PCR and immunohistochemistry.

Tags

CataractBlindnessWorld Health OrganizationClouding Of The LensMulti-factorial DiseaseDiabetesSmokingUltraviolet Radiation (UVR)AlcoholIonizing RadiationSteroidsHypertensionAnimal ModelSurgeryPrevention StrategiesApoptosisLensPharmaceuticalsRT-PCRImmunohistochemistry

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

In vivo 19F MRI for Cell Tracking

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Chemotherapy-induced Vascular Toxicity – Real-time In vivo Imaging of Vessel Impairment

Establishment and Characterization of UTI and CAUTI in a Mouse Model

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo

Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice

Read Article