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Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
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Chapitres
- 00:05Titre
- 01:06Isolation of Brain Microvessels
- 04:35Immortalizing the Brain Microvascular Endothelial Cells
- 07:22Cultivating the Brain Microvascular Endothelial Cells
- 08:13Results: Representative Images of cEND Cells
- 09:14Conclusion
This method describes how to isolate and immortalize microvascular endothelial cells from mouse brain. We describe a step-by-step protocol starting from the homogenization of brain tissue, digestion steps, seeding and immortalization of the cells. Usually, it takes about five weeks to obtain a homogenous, immortalized microvascular endothelial cell line.
Tags
Immortalized Cell LineMurine Brain Microvascular Endothelial CellsIn Vitro Blood Brain Barrier ModelParacellular BarriersBlood-brain Barrier (BBB)Astrocyte End-feetPericytesBasal MembraneBrain ParenchymaIn Vitro BBB ModelsPrimary Cell PreparationsImmortalized EC LinesNeonatal Mouse BrainReagents And SolutionsHomogenous Endothelial Cell LineCEND 4
