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Produktion af lentivirusvektorer til transduktion af celler fra det centrale nervesystem
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Chapitres
- 00:05Titre
- 01:24Packaging of Lentiviral Vectors
- 03:28Concentration and Purification of the Vectors
- 04:50Titration of the Vectors
- 06:25Transduction of Neocortical Cultures
- 07:20Transduction Results
- 08:25Conclusion
I denne protokol beskriver vi fremstilling, oprensning og titrering af lentivirale vektorer. Vi giver et eksempel lentivirale vektor-formidlet gen-levering i primære dyrkede neuroner og astrocytter. Vores metoder kan også gælde for andre celletyper<em> In vitro</em> Og<em> In vivo</em>.
Tags
Lentiviral VectorsTransducing CellsCentral Nervous SystemGene DeliveryCNSNeurological DiseasesTherapeutic ApproachesDividing CellsNon-dividing CellsTransgenesPackaging CapacityLow ToxicityBiosafetyEfficiencyReplication-defectiveSelf-inactivatingSIN Lentiviral VectorsPlasmidsLong Terminal Repeat (LTR)Strong PromoterHIV-1 Tat ProteinPackaging Signal (Ψ)Rev-responsive Element (RRE)Polypurine Tract (cPPT)Nuclear Import
