Journal
/
Médecine
/
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

DOI:
10.3791/4050-v

•

12:24 min

•

July 17, 2012

•

, , , , , , , ,
1Thayer School of Engineering,Dartmouth College, 2Department of Physics and Astronomy,Dartmouth College, 3Darmouth Medical School,Dartmouth College, 4School of Computer Science,University of Birmingham

Chapitres

  • 00:05Titre
  • 02:51Animal Preparation
  • 04:53Fluorescence Tomography System Calibration
  • 06:15Imaging Protocol
  • 08:45Image Reconstruction
  • 10:34Results: Fluorescence Reconstruction
  • 11:06Conclusion

Summary

Traduction automatique

Traduction automatique

Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.

Tags

Computed Tomography-guided Time-domain Diffuse Fluorescence TomographySmall AnimalsCancer BiomarkersPreclinical Drug DiscoveryDevelopment StudiesLight AbsorptionOptical Signal PropagationVisible WavelengthsRed And Near-infrared (near-IR) Light AbsorptionNon-elastic ScatteringFluorescent AgentsImaging SystemsLight Propagation ModelsWhole Body Three-dimensional ImagingIll-posed Nature Of Diffuse Fluorescence TomographyStabilityContrast RecoverySpatial ResolutionImage Reconstruction TechniquesFMI In Small AnimalsCharge-coupled Device (CCD)-based SystemsSensitivity Detectors

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/4050?article_type=v

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
check_url/fr/4050?article_type=v

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
check_url/fr/4050?article_type=v

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
check_url/fr/4050?article_type=v

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer
check_url/fr/4050?article_type=v

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
check_url/fr/4050?article_type=v

Disposable Dosators for Pulmonary Insufflation of Therapeutic Agents to Small Animals
check_url/fr/4050?article_type=v

Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans
check_url/fr/4050?article_type=v

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function
check_url/fr/4050?article_type=v

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
check_url/fr/4050?article_type=v

Four-Dimensional Computed Tomography-Guided Valve Sizing for Transcatheter Pulmonary Valve Replacement

Read Article