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Kortlægning Bakterielle Funktionelle Networks og spredningsveje i<em> Escherichia Coli</em> Hjælp Syntetiske Genetiske Arrays
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Journal JoVE Biologie
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Kortlægning Bakterielle Funktionelle Networks og spredningsveje i<em> Escherichia Coli</em> Hjælp Syntetiske Genetiske Arrays

DOI:
10.3791/4056-v

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14:06 min

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November 12, 2012

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, , ,
1Department of Molecular Genetics,University of Toronto, 2Banting and Best Department of Medical Research, Donnelly Centre,University of Toronto, 3Department of Biochemistry, Research and Innovation Centre,University of Regina

Chapitres

  • 00:05Titre
  • 01:40Constructing Non-essential Query Deletion in an Hfr Cavalli Donor Strain
  • 06:42Arraying E. coli F-Recipient Mutants for E. coli Synthetic Genetic Array (eSGA) Screens
  • 08:45High-density Strain Mating Procedure to Generate E. coli Double Mutants
  • 11:01Results: Representative Analyses of Genetic Interaction (GI) Scores for Determining Pathway-level Functional Relationships
  • 13:16Conclusion

Summary

Traduction automatique

Traduction automatique

Systematiske, store syntetiske genetiske (gen-gen eller epistasis) interaktion skærme kan bruges til at udforske genetiske redundans og pathway cross-talk. Her beskriver vi en high-throughput kvantitativ syntetisk genetisk array-screening teknologi, kaldet eSGA, som vi udviklede for at kunne belyse epistatic relationer og udforske genetiske interaktion netværk i<em> Escherichia coli</em>.

Tags

Bacterial Functional NetworksPathwaysEscherichia ColiSynthetic Genetic ArraysPhenotypesPhysical InteractionsFunctional InteractionsGene-gene InteractionsGenetic InteractionsGI ScreensEpistatic DependenciesFunctional RelationshipsLarge-scale GI MapsProkaryotesFunctional AnnotationBacterial GenomesQuantitative Bacterial GI Screening MethodsE. Coli Synthetic Genetic Array (eSGA) Screening ProcedureGenome-scale Screening

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