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Chapitres
- 00:05Titre
- 01:47Construction of Gene-specific SPA-tagging in E. Coli DY330 Strain
- 03:54Culturing and Sonication
- 05:26Affinity Purification
- 07:53Proteolysis and Sample Preparation for Mass Spectrometry
- 09:38Protein Identification by LTQ Orbitap Velos Mass Spectrometer
- 11:02Résultats
- 14:27Conclusion
Tags
Protein ComplexesEscherichia ColiSequential Peptide Affinity PurificationTandem Mass SpectrometryProtein-protein InteractionsSubunit CompositionGene FunctionBiological SystemsAffinity PurificationMass SpectrometryChromosomally-tagged ProteinsCarboxy-terminal Sequential Peptide Affinity (SPA) Dual Tagging SystemGram-negative Escherichia ColiGenetically-tractable Host Laboratory StrainsGenome-wide InvestigationsProkaryotesTandem Affinity Purification MethodCalmodulin Binding Peptide (CBP)Tobacco Etch Virus
