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Chapitres
- 00:05Titre
- 00:46Cultivation and Harvest of P. putida
- 01:37Formulation Solutions, Preparing Cells for Freeze-drying, and Surface Tension Measurements
- 03:22Characterization of Formulation Freezing Behavior by Differential Scanning Calorimetry (DSC) and Freeze-drying
- 05:31X-ray Analysis of the Dry Formulations and Electron Microscopy
- 06:38Representative Freeze-drying Results
- 08:37Conclusion
Frysetørring er ofte en nem og bekvem måde at opnå tørre produkter af levedygtige bakterieceller. Et spørgsmål i processen er celleoverlevelse. Vi detaljer her en fremgangsmåde til at undersøge, hvordan celleoverlevelse under frysetørring påvirkes af egenskaberne af den anvendte formulering.
Tags
FormulationsFreeze-dryingBacteriaCell SurvivalDehydration MethodMatrixProtectionSucroseFicoll PM400Hydroxyethyl CelluloseHydroxypropyl Methyl CelluloseP. Putida KT2440A. Chlorophenolicus A6RehydrationCharacterizingDifferential Scanning Calorimetry (DSC)Surface Tension MeasurementsX-ray AnalysisElectron MicroscopySurvival RatesPolymers
