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Chapitres
- 00:05Titre
- 01:38Sexual Cycling
- 06:13Flow Cytometry
- 09:26Genotyping
- 10:28Results: Yeast Strains Carrying Multiple Gene Deletions as Generated by the Green Monster Process
- 12:47Conclusion
The Green Monster method enables the rapid assembly of multiple deletions marked with a reporter gene encoding green fluorescent protein. This method is based on driving yeast strains through repeated cycles of sexual assortment of deletions and fluorescence-based enrichment of cells carrying more deletions.
Tags
Green Monster ProcessYeast StrainsGene DeletionsPhenotypesGenetic BackgroundEnvironmental ConditionMutation TestingHidden Gene FunctionsGenetic InteractionsMulti-mutant InteractionsParalogsSaccharomyces CerevisiaeGene KnockoutSelectable MarkerHomologous RecombinationMarker RemovalGreen Monster MethodGreen Fluorescent Protein (GFP) Reporter
