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Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
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Journal JoVE Biologie
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

DOI:
10.3791/4078-v

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06:10 min

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July 27, 2012

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, , ,
1Albrecht-Kossel-Institute for Neuroregeneration,School of Medicine University of Rostock, 2Institute of Anatomy I,School of Medicine University of Jena

Chapitres

  • 00:05Titre
  • 01:22Ex ovo Culture
  • 02:51Preparation for Ex ovo Electroporation
  • 03:51Gene Transfer into Chicken Optic Tectum by Ex ovo Electroporation
  • 04:43Results: Overexpression of Cad7 and GFP by Ex ovo Electroporation
  • 05:50Conclusion

Summary

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A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.

Tags

Gene TransferChicken EmbryosEx Ovo ElectroporationGene FunctionGene RegulationIn VivoDNA PlasmidsSiRNA PlasmidsRNA OligosMorpholino Antisense OligonucleotidesIncubation StagesVitelline MembraneAllantoic VesselsGene Transfer Protocol

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