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In Ovo Electroporations of HH Stage 10 Chicken Embryos
Journal JoVE
Biologie
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Journal JoVE Biologie
In Ovo Electroporations of HH Stage 10 Chicken Embryos
DOI:

13:06 min

November 01, 2007

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Chapitres

  • 00:01Titre
  • 00:28Introduction
  • 01:35Proper Egg Handling
  • 02:23Windowing the Egg
  • 04:05Injection and Electroporation
  • 11:43Conclusion

Summary

Traduction automatique

Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.

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