Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:16Introduction
- 01:58Preparation of Cell Suspension from Lymph Nodes
- 04:04Erythrocyte Lysis
- 06:13Magnetic Labelling of Cells and Setting up MACS Column
- 07:49Separation of CD4+ T cells using MACs Column
- 09:13Conclusion
Isolation of lymphocytes using the Miltenyi MACs kit is a reliable way to purify cells from whole lymphoid tissue homogenates. Cells purified using the Miltenyi system are typically ≥ 96% pure. Here, we demonstrate the steps taken to isolate CD4+ T cells, one of the many kits offered by Miltenyi.
Tags
CD4+ T CellsMouse Lymph NodesMiltenyi MACS PurificationCell IsolationMagnetic Bead-based Cell SeparationPositive SelectionNegative SelectionUntouched CD4+ Helper T CellsPurityProtocol2-Photon Imaging Of Peripheral Lymph Nodes In MiceAdoptive TransferFACS AnalysisAntibodiesNon-sterile MethodSterile Conditions
