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Automated Quantification of Synaptic Fluorescence in C. elegans
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Journal JoVE Neurosciences
Automated Quantification of Synaptic Fluorescence in C. elegans

Automated Quantification of Synaptic Fluorescence in C. elegans

DOI:
10.3791/4090-v

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12:22 min

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August 10, 2012

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,
1Department of Biological Sciences,University of Toledo

Chapitres

  • 00:05Titre
  • 01:29Preparation of Worms for Imaging
  • 04:50Confocal Imaging
  • 07:34Automated Identification and Analysis of Individual Synaptic Clusters
  • 10:28Representative Results
  • 11:58Conclusion

Summary

Traduction automatique

Traduction automatique

The abundance of neurotransmitter receptors clustered at synapses strongly influences synaptic strength. This method quantifies fluorescently-labeled neurotransmitter receptors in three dimensions with single-synapse resolution in C. elegans, allowing hundreds of synapses to be rapidly characterized within a single sample without distortions introduced by z-plane projection.

Tags

Automated QuantificationSynaptic FluorescenceC. ElegansSynapse StrengthNeurotransmitter ReceptorsSynaptic SitesPostsynaptic MembraneReceptor TraffickingSynaptic PlasticityNeuromodulationFluorescence MicroscopySynaptically-localized Neurotransmitter Receptor AbundanceSynaptic DevelopmentSynaptic PlasticityCaenorhabditis ElegansNative SynapsesAutomated IdentificationThree Dimensions

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