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C. elegans Tracking and Behavioral Measurement
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Neurosciences
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Journal JoVE Neurosciences
C. elegans Tracking and Behavioral Measurement

C. elegans Tracking and Behavioral Measurement

DOI:
10.3791/4094-v

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07:36 min

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November 17, 2012

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, , ,
1Donnelly Centre,University of Toronto, 2Department of Physics and Astronomy,Vrije Universiteit, 3Okinawa Institute of Science and Technology, 4Department of Physics,University of Toronto

Chapitres

  • 00:00Titre
  • 01:08Description of Tracking Microscope
  • 02:35Preparing Tracking Plates and C. elegans for Tracking
  • 04:03Worm Tracking
  • 05:27Data Analysis
  • 06:41Conclusion

Summary

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We have developed a video-rate tracking microscope system that can record and quantify C. elegans behavior at high resolution and high speeds. We have also developed computational methods to reduce the dimensionality of the worm images to a fundamental set of measurements that completely describe the shape of the worm.

Tags

C. ElegansTrackingBehavioral MeasurementLocomotory BehaviorImage ProcessingData AnalysisAgar PlateGenetic Basis Of BehaviorSensory BehaviorLocomotionMutational PhenotypingCamera SystemIllumination SystemMechanical StimulusTracking SystemCalibration FeatureVideo TrackingHigh Dimensional DataIndependent ComponentsWorm BehaviorReversal State

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