This content is Free Access.
Chapitres
- 00:05Titre
- 01:05Growing and Harvesting HeLa Cells for Nuclear Extraction
- 01:56Swell HeLa Cells in Hypotonic Buffer
- 03:00Lyse Cells using a Dounce Homogenizer
- 04:27Salt-extract the Nuclei
- 05:15Concentrate and Dialyze the Nuclear Extract
- 06:16Results: Small-scale Nuclear Extract Technique for Functional Assays of Gene Expression Machineries
- 07:51Conclusion
